Recently, the world's problem has become in the spread of microbes and the extent of their impact on public health, especially the problem of microorganisms causing food spoilage. The study was aimed to characterize and determine antibiogram of bacteria isolated from irrigated vegetables and their sensitivity to commonly used antibiotics was carried out in Kano metropolis which are; Fagge (Kwakwaci wastewater irrigation area), Kumbotso (Shagari quarters wastewater irrigation area) and Ungoggo (Jaba wastewater irrigation area)”. In the study, the most common bacteria isolated and identified were Staphylococcus aureus (30.43%), Escherichia coli (27.54%) followed by Salmonella (24.64%) and Shigella (17.39%). The results of molecular indicate the genus and species of each isolate. The gel electrophoresis confirmed the presence of PCR products of the expected size. Sequencing and subsequent bioinformatics analysis further validated the results. In Gram-negative bacteria, Septrin, Sparfloxacin, ciprofloxacin, amoxicillin augmentin, gentamycin, perfloxacin streptomycin were sensitive. At the same time, most of the remaining antibiotics (chloramphenicol, tarivid, levofloxacin and nitrofuraticin) are 100% resistant to bacterial isolates. However, in Gram-positive bacteria, ciprofloxacin, norfloxacin, rifampicin, erythromycin, chloramphenicol, levofloxacin, azithromycin and imipenem were sensitive while the remaining antibiotics for Gram-positive bacteria (gentamycin, amoxicillin, streptomycin, ampiclox) were resistance to bacterial isolates.
Musa et al. (Tue,) studied this question.