Figure 5 from Therapeutic Efficacy of IL7/CCL19-Expressing CAR-T Cells in Intractable Solid Tumor Models of Glioblastoma and Pancreatic Cancer

In vitro responses of autologous Conv. and 7 × 19 CAR-T against patient-derived pancreatic cancer organoids. A, Surface expression of endogenous HER2 was assessed in patient-derived pancreatic cancer organoids by flow cytometry. Open and filled histograms indicate staining with humanized anti-HER2 Ab and nonstaining, followed by anti–human IgG Ab, respectively. B–D, Anti-HER2 Conv. or 7 × 19 CAR-T or UTD was cocultured with HER2-positive pancreatic cancer organoids at an effector to target (E:T) ratio of 1:1 for 4 days. B, Morphologic images after 4 days of coculture. C, The number of residual tumor cells after coculture was analyzed by flow cytometry. Data are shown as mean ± SD of triplicate samples. D, The supernatants were harvested after 4 days of coculture, and the concentration of IFNγ was assessed by ELISA. Data are shown as mean ± SD of triplicate samples. **, P P