Figure 4 from Tumor-Intrinsic Activity of Chromobox 2 Remodels the Tumor Microenvironment in High-grade Serous Carcinoma

Overexpression of CBX2 leads to an increase in M2-like macrophage activity. A, Diagram of co-culture experimental setup with OVCAR4 CBX2 OE exposed to primary monocytes (from eight unique donors), then analyzed by flow utilizing M1- and M2-like markers. B, Flow cytometry analysis of CBX2 OE compared with control, increase in M2-like macrophages (CD163 and CD206). C and D, Plots of the fold change of M1- and M2-like receptors in control, CBX2 OE, +LPS/INFγ, and IL4/IL10/IL13, in which IL4/IL10/IL13 serves as the control for M2-like stimulation. E, Functional phagocytosis assay of monocytes co-cultured with control OVCAR4 compared with CBX2 OE. F, Functional phagocytosis assay, +LPS (M1 stimulatory) compared with +IL4, +IL10, and +IL13 (M2 stimulatory). Error bars, SEM. Statistical test, unpaired t test. Note: Each dot represents a unique PBMC donor.