Anti-angiogenic Activities of Methanol Extracts of Imbao (Anodontia edentula) Using Chorioallantoic Membrane Assay

Angiogenesis is a physiological process with a role in vessel proliferation that is significant in tumor development. Inhibition of this process is called anti-angiogenesis, employing the use of various natural medical agents. These include mollusks, which are deemed as a source of different medicinal compounds that can act as anti-angiogenesis treatments utilized in cancer therapy. This study aims to investigate different concentrations of methanol extracts of a molluscan marine bivalve, Anodontia edentula, and determine the anti-angiogenic activity of the extract using chorioallantoicmembrane (CAM) assay. By utilizing vessel count analysis, results show A. edentula extracts exhibit anti-angiogenic activity, with negative values in percent growth and positive values in percent inhibition. Two out of four concentrations of extracts (1000 and 500 ppm) exhibit no significant difference with positive control (ibuprofen) regarding its anti-angiogenic ability. It is highly recommended to investigate the compounds responsible for the angiogenic inhibition observed in A. edentula.