RSV Nanopore Whole Genome Sequencing v1

This is the Nanopore sequencing protocol used with ARTIC RSV primers to sequence RSV samples in the Royal Infirmary of Edinburgh, which has been utilised in this virological post (https://virological.org/t/preliminary-results-from-two-novel-artic-style-amplicon-based-sequencing-approaches-for-rsv-a-and-rsv-b/918). Primer scheme details can be found on the ARTIC RSV github page (https://github.com/artic-network/artic-rsv). This protocol is used for routine RSV sequencing here, but these RSV primers have been developed to slot in to any existing amplicon sequencing protocols used in your lab, and have been used successfully in a range of different labs (publication in preparation).