Nuclei Isolation from Human Synovium and Infrapatellar Fat Pad for 10x Multiome v1

This protocol describes isolation of nuclei from fresh-frozen human knee synovium and fat pad for use in Omics analyses, including RNA-sequencing of ATAC-sequencing. Tissue dissociation was the most critical step in optimization which was monitored by nuclei yield, integrity and purity. Methods for quality control are also described.