Figure S10 from HDAC6 Inhibition Releases HR23B to Activate Proteasomes, Expand the Tumor Immunopeptidome and Amplify T-cell Antimyeloma Activity

Fig. S10. Effect of proteasome inhibitors on presentation of the SIINFEKL-MHC class I molecule complex. E.G7-Ova cells were treated with pharmacologics at the indicated concentration for 16 h. Cells were then stained with a monoclonal antibody to Ova 257-264 (SIINFEKL) peptide bound to H2Kb and quantitated using a BD-LSRII sorter interfaced with FlowJo software as above.