Figure S16 from HDAC6 Inhibition Releases HR23B to Activate Proteasomes, Expand the Tumor Immunopeptidome and Amplify T-cell Antimyeloma Activity

Fig. S16. Effect of anti-HLA antibody on the generation of apoptotic MM cells after treatment with HDCA6 inhibitors and co-culture with autologous T-cells. MM patient CD138+ cells (20,000/ sample) were treated with each HDAC6 inhibitor (1 uM) for 24 h followed by incubated with 0.5 ug/mL mouse anti-HLA ABC antibody W6/32 (catalog number 14-9983-82, Fisher Scientific, Pittsburgh, PA) followed by co-culture with T-cells (E:T 2:1). The percent of apoptotic cells was quantitated by flow cytometry. Values represent the average of triplicate measurements. Error bars represent the SD of the mean.