Figure 5 from Deuterium Metabolic Imaging Differentiates Glioblastoma Metabolic Subtypes and Detects Early Response to Chemoradiotherapy

Imaging the effects of chemoradiation on glucose metabolism in tumor models representative of the glycolytic and mitochondrial subtypes. A-D, Representative spectra from voxels within the tumor and normal-appearing brain in 3D 2H chemical shift images of A11 (A and B) and S2 (C and D) tumor–bearing animals infused with 2 g/kg 6,6-2H2glucose before (A and C) and after (B and D) treatment. Fits to the spectra are shown in color: red, water; green, glucose; blue, Glx; and purple, lactate. The tumor is outlined in yellow on the T2-weighted 1H image. The locations of the CSI voxels are indicated by the boxes. The spectra are the sum of six 10-minute spectra acquired from 3 × 3 × 9 mm voxels. Labeled glucose, Glx, and lactate concentrations were determined from summed spectra. E-J, Labeled glucose (E), Glx (F), and lactate (G) concentrations in A11 tumors before and after treatment, and labeled glucose (H), Glx (I), and lactate (J) concentrations in S2 tumors before and after treatment. ns, not significant; *, P