12S rRNA-Gene Metabarcoding Library Prep: Dual-PCR Method v1

This protocol is used for eDNA metabarcoding of the mitochondrial 12S rRNA gene (Miya et al 2015) using Pair-End Illumina Miseq Sequencing. This protocol was implemented as part of the Urban Ocean Biodiversity project, a collaboration between the University of British Columbia's Institute for the Oceans and Fisheries, and Tsleil-Waututh First Nation. This protocol is developed to give a species-level resolution of fish diversity.