Evaluation of Anthelmintic Activity of Different Extracts of Azadirachta indica Flower/Buds in Indian Earthworm

Helminthiasis is a parasitic infection affected widespread among variety of living creatures amongst them humans and animals are mostly affected and the cause is helminths.The strategy of the disease is very high specifically in developing countries and the basic reason is inadequate sanitary conditions and poor management practices.Azdirachta indica (A.indica) flowers/buds extracts were prepared with different solvents in increasing order of polarity (diethyl ether < ethanol < aqueous).Stock solution of the extract of A. indica was prepared in distilled water at the concentrations of 20, 50 and 100 mg/ml and used against test worms.The present study was aimed to evaluate the biological activity with special focus on anthelmintic activity of different extracts of A. indica against Pheretima posthuma and Tubifex tubifex.The time of paralysis and time of death were studied and the activity was compared with Albendazole as reference standard and distilled water was used as control.The alcohol and aqueous extract at doses 50 mg/ml and 100 mg/ml exhibited significant anthelmintic activity as evidenced by decreased paralyzing time and death time.