Determination of Antimicrobial Activity of Extracts of Carica papaya Seed

There are a number of antimicrobial agents suggested worldwide. However, many of them exhibit unexpectedly harmful side effects on human beings. The extracts of Carica papaya seeds were prepared through the soxhlet extraction technique, and the antimicrobial activity was analyzed using the agar-well diffusion method, where dimethyl sulfoxide (DMSO) was used as a negative control while antibiotic amoxicillin was used as a positive control. The objective of the study is to evaluate the antimicrobial activity of methanolic, ethanolic, and aqueous extracts of naturally occurring seeds of C. papaya against human pathogens such as Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. The results of the study indicated that the methanolic and ethanolic extracts of C. papaya seeds showed very effective antimicrobial behavior against all the test pathogens, while the aqueous seed extracts showed lower antimicrobial behavior. The results revealed a highest zone of inhibition and antimicrobial activity against S. aureus. The present study focuses on the use of C. papaya seeds as an antimicrobial agent in treating bacterial infectious diseases. . KEYWORDS :Agar well-diffusion method, Antimicrobial activity, Carica papaya seeds