Development of a rapid isothermal amplification method for clinical detection of Pseudomonas aeruginosa

Background Pseudomonas aeruginosa (P. aeruginosa) is widely distributed in air, soil, and water, human respiratory tract, intestinal tract, and skin. It can induce bloodstream infection, urinary tract infection, gastrointestinal tract infection, respiratory tract infection, etc. Conventional bacterial isolation, culture, and identification are time-consuming, and many false negative results, which cannot meet the needs of precise clinical diagnosis,and proper treatment. This study aims to develop a rapid isothermal amplification assay of Pseudomonas aeruginosa.