Pd55-11 Assessing the Number of Spermatogonia in Pediatric Patients With Undescended Testes Using Pgp9.5 Immunohistochemical Staining

You have accessJournal of UrologyPediatrics VI (PD55)1 May 2024PD55-11 ASSESSING THE NUMBER OF SPERMATOGONIA IN PEDIATRIC PATIENTS WITH UNDESCENDED TESTES USING PGP9.5 IMMUNOHISTOCHEMICAL STAINING Megan Escott, Abinav Udaiyar, Evan Zelt, Ibrahim Hacibey, Leila Heidari, Demetri Hodges, Heather Barber, Guillermo Galdon, Nima Zarandi, Kimberly Stogner-Underwood, Shadi Qasem, Stanley Kogan, Anthony Atala, and Hooman Sadri-Ardekani Megan EscottMegan Escott , Abinav UdaiyarAbinav Udaiyar , Evan ZeltEvan Zelt , Ibrahim HacibeyIbrahim Hacibey , Leila HeidariLeila Heidari , Demetri HodgesDemetri Hodges , Heather BarberHeather Barber , Guillermo GaldonGuillermo Galdon , Nima ZarandiNima Zarandi , Kimberly Stogner-UnderwoodKimberly Stogner-Underwood , Shadi QasemShadi Qasem , Stanley KoganStanley Kogan , Anthony AtalaAnthony Atala , and Hooman Sadri-ArdekaniHooman Sadri-Ardekani View All Author Informationhttps://doi.org/10.1097/01.JU.0001008908.82706.9f.11AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: The quantity of spermatogonia can be affected by radiation, chemotherapy, environmental exposures, or genetic disorders. Pathology scores such as Kretser-Holstein scores evaluate spermatogenic cells in adult testes and are based on various stages of spermatogenic cells in seminiferous tubules. These scoring systems, however, are not valuable for pediatrics as spermatogonia is the only germ cell in immature testes. We previously reported preliminary data on spermatogonia quantity in normal pediatric patients compared to a small cohort with undescended testes (UDT). Here we report the more extensive, final cohort. METHODS: Biopsies from patients with no testicular pathology were acquired through the Atrium Health Wake Forest Baptist Testicular Tissue Bank, an IRB-approved experimental fertility preservation program for children needing chemotherapy for cancer outside of the testis. Participants with a history of bilateral UDT were also eligible for inclusion. Testicular sections were stained with PGP9.5 (UCHL1) antibody, an undifferentiated spermatogonial marker, and processed in an automated clinical setting. The numbers of basal and adluminal spermatogonia were manually counted on slides in a digital viewing software. RESULTS: 84 normal and 61 UDTs were evaluated (age 2 months – 12 years). Table 1 reports the average spermatogonia quantity in patients with no testicular pathology. When compared to UDT across all ages, the average number of total spermatogonia was significantly higher in normal testes (3.42 vs. 1.27, p<0.0001). Normal testes also had significantly higher numbers of basal and adluminal spermatogonia (2.69 vs. 0.92, p<0.0001 and 0.73 vs. 0.33, p<0.002 respectively). Significant differences in spermatogonia quantity remain present in normal and UDT when cohorts were stratified into pre-pubertal (<10 years old) and peri-pubertal ages (10 to 12 years old). CONCLUSIONS: We describe a clinically validated method to quantify the spermatogonia present in pediatric patients. Patients with UDT had lower spermatogonia counts than their age-matched counterparts. Future work is needed to validate pediatric spermatogonia assessment and improve clinicians' ability to quantify a pediatric patient's risk of infertility and provide appropriate counseling. Source of Funding: Institutional (Internal) Funding © 2024 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e1154 Advertisement Copyright & Permissions© 2024 by American Urological Association Education and Research, Inc.Metrics Author Information Megan Escott More articles by this author Abinav Udaiyar More articles by this author Evan Zelt More articles by this author Ibrahim Hacibey More articles by this author Leila Heidari More articles by this author Demetri Hodges More articles by this author Heather Barber More articles by this author Guillermo Galdon More articles by this author Nima Zarandi More articles by this author Kimberly Stogner-Underwood More articles by this author Shadi Qasem More articles by this author Stanley Kogan More articles by this author Anthony Atala More articles by this author Hooman Sadri-Ardekani More articles by this author Expand All Advertisement PDF downloadLoading ...