Mp66-14 Markers of Neovascularization and Neurogenesis May Change After Low-Intensity Extracorporeal Shockwave Therapy for Erectile Dysfunction

You have accessJournal of UrologySexual Function/Dysfunction: Basic Research & Pathophysiology (MP66)1 May 2024MP66-14 MARKERS OF NEOVASCULARIZATION AND NEUROGENESIS MAY CHANGE AFTER LOW-INTENSITY EXTRACORPOREAL SHOCKWAVE THERAPY FOR ERECTILE DYSFUNCTION Ashley Matthew, Skye Coffey, Tu Nguyen, Paulene Sapao, Adam P. Klausner, Ryan P. Smith, Maria Teves, and Sarah C. Krzastek Ashley MatthewAshley Matthew , Skye CoffeySkye Coffey , Tu NguyenTu Nguyen , Paulene SapaoPaulene Sapao , Adam P. KlausnerAdam P. Klausner , Ryan P. SmithRyan P. Smith , Maria TevesMaria Teves , and Sarah C. KrzastekSarah C. Krzastek View All Author Informationhttps://doi.org/10.1097/01.JU.0001009468.01097.19.14AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Low-intensity extracorporeal shockwave therapy (LiESWT) is thought to treat ED by stimulating neovascularization and nerve regeneration as demonstrated in animal models by histology. Given its prolific use, a thorough understanding of its mechanism of action at a cellular level is critical. Here we aimed to determine whether markers for neovascularization and nerve regeneration can be detected in the plasma of human penile tissue following LiESWT. We hypothesized that LiESWT increases these factors as detectable by Enzyme-Linked Immunosorbent Assay (ELISA). METHODS: Patients were prospectively enrolled and received 12 bi-weekly LiESWT treatments of 0.2 mJ/mm2 at 5Hz, 1500 shocks per treatment, with follow up at 1-2 weeks, 4-6 weeks, 3 months and 6 months post-treatment. Cavernosal blood samples were obtained prior to treatment and at each visit post-treatment. Plasma concentrations of BDNF, VEGF, eNOS, and nNOS were measured using ELISA with commercial kits per manufacturer protocol. RESULTS: Twenty-five patients were enrolled. Mean patient age was 63. Mean baseline IIEF-EF score prior to treatment was 14.24 (±1.21). Plasma samples were analyzed for BDNF, VEGF, eNOS, and nNOS using ELISA. BDNF levels decreased with time with significantly lower levels at 6 months (Figure 1A). Levels of VEGF, nNOS and particularly eNOS trended up but did not reach significance. CONCLUSIONS: To our knowledge, this is the first study in humans to assess neurogenesis and neovascularization in penile tissue following LiESWT for ED. Though our N is small, our results suggest that LiESWT may decrease BDNF, eNOS may increase, and VEGF and nNOS remain unchanged. The clinical significance of these findings is unknown. Our findings are not consistent with histological studies in animals demonstrating consistent upregulation of these markers. However, we believe this is a promising first step in understanding the effect of LiESWT at a tissue level. Download PPT Source of Funding: Research funded by McGuire Research Institute/Virginia Commonwealth University Joint Pilot Grant Fund, Richmond, VA. Storz Duolith T-Top Ultra Low-Intensity Extracorporeal Shockwave machine donated by the Virginia Commonwealth Division of Urology Endeavour Legacy Foundation © 2024 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e1091 Advertisement Copyright & Permissions© 2024 by American Urological Association Education and Research, Inc.Metrics Author Information Ashley Matthew More articles by this author Skye Coffey More articles by this author Tu Nguyen More articles by this author Paulene Sapao More articles by this author Adam P. Klausner More articles by this author Ryan P. Smith More articles by this author Maria Teves More articles by this author Sarah C. Krzastek More articles by this author Expand All Advertisement PDF downloadLoading ...