Mp66-20 Endothelial Function and Pde5-Inhibitor-Induced Responses in Corpus Cavernosum From Patients With Erectile Dysfunction Are Improved by Acute Exposure to Autologous Platelet-Rich Plasma

You have accessJournal of UrologySexual Function/Dysfunction: Basic Research & Pathophysiology (MP66)1 May 2024MP66-20 ENDOTHELIAL FUNCTION AND PDE5-INHIBITOR-INDUCED RESPONSES IN CORPUS CAVERNOSUM FROM PATIENTS WITH ERECTILE DYSFUNCTION ARE IMPROVED BY ACUTE EXPOSURE TO AUTOLOGOUS PLATELET-RICH PLASMA Esaú Fernández Pascual, Raphael Curvo, Argentina Fernández, Claudio Martínez-Ballesteros, José Luis Bueno, Javier Angulo, and Juan Ignacio Martínez-Salamanca Esaú Fernández PascualEsaú Fernández Pascual , Raphael CurvoRaphael Curvo , Argentina FernándezArgentina Fernández , Claudio Martínez-BallesterosClaudio Martínez-Ballesteros , José Luis BuenoJosé Luis Bueno , Javier AnguloJavier Angulo , and Juan Ignacio Martínez-SalamancaJuan Ignacio Martínez-Salamanca View All Author Informationhttps://doi.org/10.1097/01.JU.0001009468.01097.19.20AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVE: Intracavernosal injections of platelet-rich plasma (PRP) have been proposed as a potential therapeutic tool for treating erectile dysfunction (ED). However, the real impact of PRP on human cavernosal function has not been elucidated. Our aim was to evaluate the effects of acute exposure to autologous PRP on relaxant capacity of human corpus cavernosum (HCC) from patients with ED. METHODS: HCC strips were obtained from six ED patients undergoing penile prosthesis insertion and giving informed consent. Before surgery, PRP and platelet-poor plasma (PPP) were obtained from patients by apheresis and stored at -20°C until functional procedures. Endothelial relaxation to acetylcholine (ACh) was evaluated before and after 60 min treatment with PRP or PPP (1% v/v) in organ chambers. Relaxant response to the PDE5 inhibitor, tadalafil, was also evaluated in PRP or PPP (1%) treated HCC from the same patient. RESULTS: Patients' mean age was 65.8±2.6 years while hypertension was present in 5/6, diabetes in 3/6, obesity in 1/6 and smoking history in 4/6. Mean platelet content in PRP was 1,027±93x109/L while all PPPs had negligible platelet content. Exposure to autologous PRP and PPP enhanced ACh-induced endothelial relaxations but the improvement was larger with PRP, reaching a significant increase in pEC50 for ACh (ΔpEC50 0.08±0.05, 0.60±0.30 and 1.49±0.33 for vehicle, PPP 1% and PRP 1%, respectively, n=6, p<0.0001 PRP vs. PPP). Higher platelet content in PRP was not related to better response. Heterologous PRP at 1% also enhanced ACh-induced relaxations but the effect was smaller than autologous (ΔpEC50 0.61±0.12, n=4, p=0.0103). Relaxant responses to tadalafil were significantly potentiated in HCC treated with autologous PRP with respect to those treated with PPP (p=0.0381). CONCLUSIONS: Acute treatment of HCC with autologous PRP improves endothelial function and response to PDE5-inhibition. This suggests that independently on claimed restorative/regenerative effect, PRP injection might induce direct functional effects on cavernosal tissue contributing to the reported clinical improvement of erectile function by PRP in patients with ED. Source of Funding: Ministry of Economy and Competitiveness and co-financed by FEDER funds, Instituto de Salud Carlos III, PI17/02001, Spanish Government © 2024 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 211Issue 5SMay 2024Page: e1094 Advertisement Copyright & Permissions© 2024 by American Urological Association Education and Research, Inc.Metrics Author Information Esaú Fernández Pascual More articles by this author Raphael Curvo More articles by this author Argentina Fernández More articles by this author Claudio Martínez-Ballesteros More articles by this author José Luis Bueno More articles by this author Javier Angulo More articles by this author Juan Ignacio Martínez-Salamanca More articles by this author Expand All Advertisement PDF downloadLoading ...