ARTIC SARS-CoV-2 sequencing protocol v4 (LSK114) v2

Amplicon sequencing protocol for SARS-CoV-2 v4 (LSK114) We thank the ARTIC network, Oxford Nanopore Technologies, New England Biolabs, BCCDC, COG-UK, CanCOGen and protocols.io commenters for their assistance developing this protocol. Changes in this version: -Up to 95 samples per run with EXP-NBD196 native barcode kit -Substitution of SuperScript IV for LunaScript RT SuperMix and reaction volume reduced to 10 uL. -Substitution of Ultra II Ligation Module for Blunt/TA Ligase Master Mix and reaction volume reduced to 10 µL. -Native barcode ligation reaction volume reduced to 10 uL. -SFB wash volume reduced.