Supplementary Figure S8 from Human Tumor–Associated Macrophages and Neutrophils Regulate Antitumor Antibody Efficacy through Lethal and Sublethal Trogocytosis

Supplementary Figure S8. (A and B) Representative experiment and summary results showing the effect of blood and tumor FcR+ effectors on the growth of GFP+A549 tumor cell spheroids in the presence of cetuximab (1ug/ml). Experiments were performed as described in supplemental figure 5 (A and B). Original magnification x10. Scale bar, 400 µm. Data are represented as mean ± SEM. Wilcoxon matched pairs test (n=6). Indicated FcR+ effectors were freshly isolated for all experiments. (C) Flow cytometry histogram showing the expression of EGFR on the surface of H1975 tumor cell line in comparison with A431 and A549 tumor cells. (D and E) Cumulative flow cytometry results showing the different levels of trogocytosis and killing activity mediated by blood and tumor FcR+ effectors co-cultured with PKH67+ H1975 tumor cells at a 50:1 E:T ratio in the presence of cetuximab or isotype control Abs for 12 hrs. All comparisons used one-way ANOVA with Turkey’s multiple comparisons tests. All data represented as mean ± SEM. FcR+ effectors were freshly isolated for all experiments. IC-isotype control Ab.