Evaluation of anti-urolithiatic activity of seed extract of Cuminum cyminum

Abstract Urolithiasis or kidney stone disease is a urologic ailment that has a high prevalence rate worldwide and medicinal plants have been widely used for alternative therapy. A wide range of medicinal plants have been used conservatively for urolithiasis due to their fewer side effects and because they contain copious phytochemicals that show advantageous effects in urolithiasis. Cuminum cyminum traditionally used in the treatment of urolithiasis. Hyperoxaluria and hypercalciuria are among the major risk factors in pathogenesis of urinary stone formation. Evaluation of various medicinal plants are done mainly against calcium oxalate. In this present study, the antiurolithiatic potential in Cuminum cyminum was investigated through invitro assay. The work was performed by using invitro Anti-urolithiatic model for calculating percentage in comparison with standard drug cystone. The anti-urolithiatic properties were evaluated by titrimetric and turbidity assay. The result shows that for C. cyminum folds (55%) and for standard drug cystone (82%) for titrimetric assay. For turbidity assay. C. cyminum has significant properties (47.9±19%). The findings of the nucleation assay indicate that phytoconstituents inhibited the crystallization of CaOx in solution. The size and the number of calcium oxalate crystals decreased with increasing concentration upto 400μg of the phytoconstituents. The study concludes that the seed extracts of Cuminum cyminum have inhibitory effect on calcium oxalate for crystal nucleation. It also showed great efficacy in the dissolution of calcium oxalate crystals. Thus, this extract may be valuable resources for treatment of urolithiasis. Acute kidney injury (AKI) is a severe problem for healthcare professionals due to its high mortality rate. The major causes of AKI are ischemia, hypoxia, and drug-induced nephrotoxicity. AKI is particularly related to an imbalance between oxygen and nutrients, which is caused by impaired circulation to the nephrons and increased energy requirement due to its oxidative stress. In vitro nephroprotective studies of Cuminum cyminum reveals promising result. Cisplatin is a highly effective chemotherapeutic agent; its clinical use is severely limited by serious side effects as nephrotoxicity. The purpose of this study is to evaluate the nephroprotective activity of ethanolic extract of Cuminum cyminum. The nephroprotective activity was dose dependent.