March 6, 2024Open Access

DOH Workshop Protocol Parts 1, 2 and 3 v1

VQVesa QarkaxhijaRoslin Institute BWBryan A. WeeRoslin Institute

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Resumen

This collection of protocols details the extraction of high-molecular-weight DNA from Gram-negative bacterial culture, purification of dsDNA, removing contaminants and low molecular weight DNA, and creation of Nanopore sequencing libraries for the MinION flow cell.

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Qarkaxhija et al. (Wed,) studied this question.

synapsesocial.com/papers/68e758b6b6db6435876d00ad https://doi.org/https://doi.org/10.17504/protocols.io.q26g7pqpkgwz/v1

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Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

1DOH Workshop Part 2: Promega Pronex protocol v12024
2DOH Workshop Protocol Part 1: Purification of High- Molecular Weight Genomic DNA from Gram-Negative Bacteria (MagAttract HMW DNA) v12024
3DOH Workshop Protocol Part 3: Library preparation for Rapid Sequencing DNA V14 Barcoding kit (SQK-RBK114.24) with Pronex modification v12024
4<i>FindingNemo</i> : A Toolkit for DNA Extraction, Library Preparation and Purification for Ultra Long Nanopore Sequencing2024 · 4 citations
5FindingNemo (v.kit14): A Toolkit for DNA Extraction, Library Preparation and Purification for Ultra Long Nanopore Sequencing v12024 · 2 citations

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