Bisphenol A (BPA; 4-2- (4-hydroxyphenyl) propan-2-yl, being an important industrial raw material, is a prevailing endocrine disruptor presenting a serious risk to human health and aquatic life. Classical Treatment methods are often inefficient or difficult to implement due to their persistent nature. Soybean peroxidase, being an iron-porphyrin-based metalloprotein, provides an efficient and eco-friendly alternative for degrading BPA. The present study involves purified soybean peroxidase (SBP) as a green catalyst to remove BPA from wastewater using response surface methodology (RSM) with six variables at five levels. The optimum conditions, which included 20 U/mL SBP, 30 mg/L H 2 O 2, a 150-minute reaction time, 40 °C, and a pH of 7. 0, resulted in 100% BPA mineralization, as confirmed by UV-Vis, HPLC, and LC-MS/MS. Kinetic analysis \ (\: V₌₀ₗ\) = 28. 6 µM/min and \ (\: K₌\) = 13. 01µM indicated high catalytic power and strong substrate affinity. The cytotoxicity study demonstrated a reduction in red blood cell (RBC) lysis from 47% to 3% of SBP-treated water. The phytotoxicity analysis revealed 0% (untreated wastewater) versus 100% (SBP-treated water) germination of wheat. This study established SBP as a cost-effective, sustainable, and eco-friendly biocatalyst for advanced and efficient treatment of micropollutants and endocrine-disrupting chemicals (EDCs) such as BPA.
Malik et al. (Mon,) studied this question.