Multidrug resistance genes (MDRG) overexpression represents a critical factor contributing to chemotherapeutic resistance in cancer, necessitating the development of highly sensitive detection technologies for early diagnosis. Herein, we synthesized a heterometallic decanuclear CeIII-WVI cluster incorporated antimonotungstate pentamer Na16H7Ce4W6O13(H2O)12B-α-SbW9O335·40H2O (1) via the reaction of the B-α-SbW9O339- precursor with Ce3+ cations in CH3COOH-CH3COONa buffer system. Its polyanionic architecture comprises a heterometallic decanuclear Ce4W6O13(H2O)622+ core encapsulated by five B-α-SbW9O339- units. Notably, 1 represents the first reported pentamer within the rare-earth incorporated antimonotungstate family. By employing 1 as a multifunctional dopant, an electroconductive 1@PNMPy film was fabricated through electropolymerization of N-methylpyrrole (NMPy), demonstrating enhanced conductivity and stability. The resulting 1@PNMPy-based electrochemical DNA biosensor (1-ECDB) achieves sensitive detection of MDRG, exhibiting a broad linear response range (1.0 × 10-14 to 1.0 × 10-7 M) and a low detection limit (4.33 fM). Moreover, the 1-ECDB exhibits acceptable specificity, anti-interference capability, reproducibility and long-term stability. Its practical applicability has been validated in diluted human serum samples, yielding recovery rates ranging from 94.2 to 109.2%. This work pioneers the application of POM-conductive polymer composite films in the electrochemical detection of MDRG, bridging the molecular engineering of POMs with biomedical sensing technologies.
Li et al. (Wed,) studied this question.