What question did this study set out to answer?

This research investigates how extended storage affects platelet function test results in blood samples.

February 17, 2026

Effects of extended storage of blood samples on the results of platelet function testing: an in vitro stability study

Resultado clave

Blood samples can be stored up to 5 hours at room temperature without affecting Collagen/ADP, ASPI, ADP, TRAP, Aspirin, and PRU test results, but Collagen/EPI shows 35% deviation.

Puntos clave

This research investigates how extended storage affects platelet function test results in blood samples.
Collected blood samples from 30 patients.
Tested platelet function using PFA-200, Multiplate, and VerifyNow analyzers.
Analyzed samples immediately, then after 3-4 hours and 5 hours of storage.
Platelet function remained stable for up to 5 hours for most tests, except Collagen/EPI.
Collagen/EPI test results deviated significantly after 5 hours with a median deviation of 35%.
No significant differences were found between 4 and 5 hours post-collection.

PICO estructurado

Does extended storage of blood samples up to 5 hours affect the results of platelet function testing?

Población

Blood samples from 30 patients

Intervención

Storage of blood samples for 5 h at room temperature (extended storage)

Comparador

Immediate testing (baseline) and storage for 3-4 h at room temperature (maximum permitted by manufacturers)

Resultado

Platelet function stability (median relative deviation from baseline values <19% or <28% depending on the test)surrogate

Extended storage of blood samples up to 5 hours at room temperature is acceptable for most platelet function tests, but not for the PFA-200 Collagen/EPI test.

Resultado numérico

Tasa de eventos absoluta: 0% vs 0%

Resumen

In the present study, we aimed to explore the effects of extended storage of blood samples from 30 patients on the results of platelet function testing using the PFA-200 device (Collagen/EPI and Collagen/ADP tests), Multiplate instrument (ASPI, ADP, and TRAP tests), and VerifyNow analyzer (Aspirin and PRU tests). We determined platelet function (1) immediately after blood collection (baseline), (2) after storage of blood samples at room temperature for 3-4 h (i.e. the maximum sample storage period permitted by the manufacturers), and (3) after storage of blood samples for 5 h at room temperature (extended storage). Per the concept of acceptable change limits, platelet function was considered stable in time course if the median relative deviation from baseline values at a given time was <19% or <28%, depending on the test used. By applying our analyte stability limits, we found that blood samples could be stored for up to 5 h when performing the Collagen/ADP, ASPI, ADP, TRAP, Aspirin, and PRU tests. However, we observed significant deviations in the Collagen/EPI test results over time. The PFA-200 Collagen/EPI test results showed a median percentage deviation of 35% after 5 h of storage. However, no significant differences were observed between the measurements taken after 4 and 5 h of storage. We assume, that the baseline values of the Collagen/EPI test are measured incorrectly (presumably owing to malfunction of the test cartridges) and the following results are measured correctly.

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