What question did this study set out to answer?

The aim is to isolate and characterize a new strain of Streptomyces capable of degrading the herbicide 2,4-D.

February 26, 2026Open Access

Multilocus sequence typing and genomic characterization of the novel Streptomyces strain SCPE-10, that resists 2,4-D toxicity and has bioremediation potential

Puntos clave

The aim is to isolate and characterize a new strain of Streptomyces capable of degrading the herbicide 2,4-D.
Isolated a novel Streptomyces strain from contaminated coastal soil.
Conducted phenotypic assays to assess growth on aromatic substrates.
Performed whole-genome sequencing and multilocus sequence analysis.
Analyzed functional genomes for genes associated with aromatic degradation.
Utilized KEGG and antiSMASH analyses to identify metabolic pathways and gene clusters.
Streptomyces sp. SCPE-10 shows robust growth using 2,4-D as the sole carbon source.
Identified a high abundance of degradation-related genes, including cytochrome P450s and dehalogenases.
Found 28 biosynthetic gene clusters for various metabolites.
Demonstrated potential for broad-spectrum degradation of aromatic compounds like benzoate and naphthalene.

Resumen

Abstract The widespread use of aromatic herbicides such as 2,4-dichlorophenoxyacetic acid (2,4-D) has led to persistent environmental contamination, requiring efficient and sustainable biodegradation strategies. In this study, we isolated and characterized a novel actinobacterial strain, Streptomyces sp. SCPE-10, from contaminated coastal soil, capable of using 2,4-D as its sole carbon source. Phenotypic assays revealed robust growth on aromatic substrates, while whole-genome sequencing (Submission no. SUB15461668 ) followed by multilocus sequence analysis (16 S rRNA, recA, rpoB, atpD, gyrB, and trpB) revealed that SCPE-10 is closely related to Streptomyces phaeoluteichromatogenes . Functional genome annotation revealed a high abundance of genes involved in aromatic compound degradation, including cytochrome P450 monooxygenases, ring-cleaving dioxygenases, and dehalogenases. KEGG and antiSMASH analyses identified multiple metabolic pathways and 28 biosynthetic gene clusters (BGCs), including clusters for polyketides, nonribosomal peptides, terpenes, siderophores, and ectoine. Notably, SCPE-10 harbors key genes related to the degradation of benzoate, naphthalene, toluene, xylene, and 2,4-D, indicating broad-spectrum catabolic potential. These findings suggest that Streptomyces sp. SCPE-10 is a promising candidate for the bioremediation of herbicide-contaminated environments and the exploration of novel secondary metabolites.

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