Alpha-glucosidase of Manduca sexta facilitates entry of Daphnis nerii cypovirus-23 by mediating virus attachment and internalization into host cells.
MsAGL acts as an entry factor for DnCPV-23 by interacting with VP3, providing insights into cypovirus cell entry mechanisms.
Tasa de eventos absoluta: 0% vs 0%
Daphnis nerii can severely damage pine forests worldwide. Daphnis nerii cypovirus-23 (DnCPV-23) is an important viral pathogen for controlling D. nerii. However, the mechanism underlying DnCPV-23 cell entry has not been elucidated. In this study, we determined that VP3 mediates the binding of DnCPV-23 to host brush border membrane vesicles. Far-Western blotting and mass spectrometry results revealed that a Manduca sexta alpha-glucosidase (MsAGL) can interact with VP3. The interaction between MsAGL and VP3 was verified by co-immunoprecipitation and glutathione S-transferase pull-down assays. Notably, MsAGL influenced DnCPV-23 entry into host cells, including attachment and the subsequent internalization of the virus. Furthermore, MsAGL inhibited DnCPV-23 infections of M. sexta cells and D. nerii larvae. In summary, we confirmed that VP3 of DnCPV-23 mediates cell entry, while also identifying MsAGL as an entry factor for DnCPV-23. The study findings provide useful insights relevant for further elucidating the cell entry mechanisms of cypoviruses.
YANG et al. (Sat,) reported a other. Alpha-glucosidase of Manduca sexta facilitates entry of Daphnis nerii cypovirus-23 by mediating virus attachment and internalization into host cells.