Single-cell metabolic profiling of Saccharomyces cerevisiae via Raman tweezers and non-negative matrix factorization: Resolving intracellular components and heterogeneity

Puntos clave

• The study identifies distinct intracellular components within individual yeast cells, indicating metabolic diversity.
• Key evidence shows that Raman tweezers effectively isolate and analyze metabolites of single cells across multiple conditions.
• Analysis utilizes non-negative matrix factorization, allowing for detailed characterization of metabolic profiles in Saccharomyces cerevisiae.
• These findings highlight the importance of single-cell analysis in understanding metabolic heterogeneity, aiding future research.