Ultra-highly specific and visual detection of Vibrio pathogens in Porphyra using RPA-coupled CRISPR-LbaCas12a-LFD system

Puntos clave

• The RPA-coupled CRISPR-LbaCas12a-LFD system achieves ultra-high specificity in detecting Vibrio pathogens.
• Detection was shown to be effective in samples of Porphyra, demonstrating a significant advancement in pathogen identification.
• This analysis utilizes cutting-edge molecular techniques to enhance pathogen detection accuracy and speed, addressing previous limitations.
• The findings highlight the potential for practical applications in food safety and environmental monitoring, emphasizing the need for reliable detection methods.