Detection and localization of single-nucleotide mutations in synthetic oligonucleotides by ultra-high-performance liquid chromatography coupled with high-resolution tandem mass spectrometry

Puntos clave

• Single-nucleotide mutations were effectively detected and localized using advanced mass spectrometry techniques.
• The analysis demonstrated the ability to identify mutations in synthetic oligonucleotides with high specificity.
• Assessment employs ultra-high-performance liquid chromatography in conjunction with high-resolution tandem mass spectrometry for accuracy.
• This innovative approach may enable more precise genetic analysis in future studies, providing deeper insights.