A self-cleavable ULP1-SUMO fusion strategy for enhanced soluble protein expression and one-step purification in Escherichia coli

Puntos clave

• Enhanced soluble protein expression is achieved using a self-cleavable ULP1-SUMO fusion strategy, and this simplifies purification.
• The method allows for one-step purification of proteins, streamlining the process compared to traditional methods.
• Assessment reveals significant improvements in yield and purity of expressed proteins across various tests.
• These findings highlight potential applications in biotechnology and pharmaceutical industries, where efficient protein production is critical.