M.O.C.: With no relation to this manuscript, Dr. Christensen has been a sub-investigator for LEO Pharma, paid consultant for LEO Pharma, and paid speaker for Pierre Fabre. S.F.T.: With no relation to the present manuscript, Dr. Thomsen has been a speaker or has served on advisory boards for Sanofi, AbbVie, LEO Pharma, Pfizer, Eli Lilly, Novartis, UCB Pharma, Union Therapeutics, Almirall, and Janssen Pharmaceuticals, and has received research support from Sanofi, Almirall, AbbVie, LEO Pharma, Novartis, UCB Pharma, and Janssen Pharmaceuticals. J.P.T.: An advisor for AbbVie, Almirall, Arena Pharmaceuticals, Coloplast, OM Pharma, Aslan Pharmaceuticals, Union Therapeutics, Eli Lilly & Co, LEO Pharma, Pfizer, Regeneron, and Sanofi-Genzyme, a speaker for AbbVie, Almirall, Eli Lilly & Co, LEO Pharma, Pfizer, Regeneron, and Sanofi-Genzyme, and received research grants from Pfizer, Regeneron, and Sanofi-Genzyme. Dr. Thyssen is a full-time employee at Leo Pharma. D.E.M.: A full-time employee at Leo Pharma. C.M.O.: Employed as industrial postdoc at LEO Pharma and Bispebjerg Hospital. The data that support the findings of this study are available on request from the corresponding author. The data are not publicly available due to privacy or ethical restrictions. Figure S1: Study design (A) Randomisation of sites for tape stripping. A, B, C and D mark the four sites for tape stripping, as well as TEWL and confocal microscopy. Site A corresponds to baseline with no tape-stripping, while 10, 30 and 50 tapes were taken from site B, C and D, respectively. Sixteen randomisation notes were prepared, each containing a full assignment of all four sample sites (T0, T10, T30, T50) across the four predefined anatomical localisations: thenar region, hypothenar region, distal to the thenar region, and distal to the hypothenar region. Each note specified the complete sampling configuration for a participant, ensuring that no participant received the same anatomical localisation for more than one intervention. Four notes placed T0 at the thenar region, 4 notes placed T0 at the hypothenar region, 4 notes placed T0 distal to the thenar region, and four notes placed T0 distal to the hypothenar region. Within each note, the remaining interventions (T10, T30, T50) were assigned to the other three anatomical sites using a clockwise rotation. The location for each participant was allocated by drawing localisation note from a box (8 for patients, and 8 for healthy). This approach ensured equal distribution of all four sampling interventions (T0, T10, T30, T50) across the four anatomical locations while preserving randomisation at the participant level. Figure S2: Visual assessment of removal of stratum corneum by RCM. RCM images at 6 μm depths after 0, 10, 30 and 50 tape-strips of dorsal hand skin in a healthy participant. (A + B) After 0 and 10 tape-strips, stratum corneum appears as a hyperreflective layer. (C) After 30 tape-strips, some areas present with a honeycomb pattern corresponding to stratum granulusom. (D) After 50 tape-strips, a honeycomb pattern are present at the entire surface, reflecting that stratum corneum is completely removed. Table S1: Demographics. Table S2: Epidermal thickness and transepidermal water loss following tape-stripping. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Yüksel et al. (Thu,) studied this question.