Introduction: Tephrosia purpurea (L.) Pers. ( Sharpunkha ) is an important medicinal herb used in Ayurveda for liver disorders, inflammation, and metabolic diseases. The Panchang (whole plant) formulation is traditionally considered therapeutically effective due to the synergistic action of multiple phytoconstituents. However, variations in phytochemical composition require reliable analytical methods for quality control and standardization. Methods: The present study aimed to standardize T. purpurea Panchang using physicochemical parameters and High-Performance Thin-Layer Chromatography (HPTLC). The plant material was authenticated, shade-dried, and powdered. Physicochemical parameters such as total ash, acid insoluble ash, loss on drying, and pH were determined according to WHO and Ayurvedic Pharmacopoeia guidelines. Preliminary phytochemical screening was performed on aqueous and methanolic extracts. HPTLC analysis was developed for fingerprint profiling of rutin, quercetin, and β-sitosterol, along with quantitative estimation of lupeol. The analytical method was validated for linearity, precision, accuracy, and sensitivity. Results: Physicochemical analysis showed total ash (9.77%), acid-insoluble ash (2.27%), loss on drying (5.09%), and pH (5.85). Phytochemical screening confirmed the presence of flavonoids and saponins. HPTLC analysis produced characteristic bands for rutin (Rf 0.32), quercetin (Rf 0.55), and β-sitosterol (Rf 0.44). Lupeol was quantified at 1.54 µg/mg of dry extract with good linearity ( r ² = 0.9987), precision (RSD < 2%), and recovery (99.3%). Conclusion: The developed HPTLC method combined with physicochemical parameters provides a reliable approach for authentication and quality control of T. purpurea Panchang.
Goyal et al. (Tue,) studied this question.
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