This study evaluated the effects of replacing trash fish (TF) with formulated feeds in tiger puffer ( Takifugu rubripes ) on the growth performance, liver and serum biochemical indices, flesh quality, and metabolomics. Fish (39.6 ± 0.2 g) were fed TF, commercial feed (CF), and self‐formulated feed (SF) for 8 weeks. Results indicated that CF and SF groups had significantly lower feed conversion ratio (FCR) and feed intake (FI) and higher protein efficiency ratio (PER) compared to TF group ( p <0.05). CF and SF groups exhibited lower flesh protein and collagen contents and shear force than TF group, with CF group showing lower flesh hardness, chewiness, and catalase (CAT) activity, and SF group showing higher superoxide dismutase (SOD) activity and lower malondialdehyde (MDA) content ( p < 0.05). Compared to TF group, serum aspartate aminotransferase (AST) and alkaline phosphatase (ALP) activities were reduced and liver triglyceride (TG) and cholesterol (CHO) contents were increased in SF group ( p < 0.05). SF and CF groups exhibited significantly lower levels of lysine (Lys), glycine (Gly), proline (Pro), cysteine (Cys), and total amino acids (TAAs) and higher flavor amino acids (FAAs) level in flesh ( p <0.05). Moreover, SF group significantly increased contents of C18:2 n −6 and n −6 polyunsaturated fatty acids ( n −6 PUFAs) and decreased n −3/ n −6 ratio ( p <0.05). Metabolomic analysis identified altered amino acid and lipid metabolism pathways. In summary, formulated feeds can effectively replace TF in tiger puffer diets; however, further optimization of amino acid and fatty acid profiles is required to improve flesh quality and nutritional value.
Xu et al. (Thu,) studied this question.
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