Abstract Summary Hydrogen–Deuterium Exchange Mass Spectrometry (HDX-MS) monitors deuterium uptake at the peptide level, in a time-dependent manner. It produces complex, multi-dimensional data that must be interpreted at minimum both the temporal and sequence levels. Specialized tools are therefore essential to preprocess, integrate, and analyze HDX-MS data and translate it into meaningful biological insights. HaDeX2 provides statistical inferences and their visualizations across five dimensions of HDX-MS data: protein sequence, time, biological states, peptide charge and experimental replicates. Availability and implementation HaDeX2 is freely available as an R package (https://github.com/hadexversum/HaDeX2; https://doi.org/10.5281/zenodo.18543703) and web server (https://hadex2.mslab-ibb.pl/). To run the GUI locally, users should install a dedicated companion package (https://github.com/hadexversum/HaDeXGUI). Supplementary information Supplementary data are available at Bioinformatics online.
Puchała et al. (Wed,) studied this question.