What question did this study set out to answer?

Investigate how Xinfeng Capsule affects inflammation and bone metabolism via the PI3K/AKT pathway in osteoarthritis rats.

March 18, 2026

Mechanism ofitalic Xinfeng Capsule/italic （新风胶囊） in regulating the PI3K/AKT signaling pathway to alleviate inflammation and bone metabolism disorder in osteoarthritis rats

Puntos clave

Investigate how Xinfeng Capsule affects inflammation and bone metabolism via the PI3K/AKT pathway in osteoarthritis rats.
Used 64 SD rats divided into 8 groups: control, model, XFC at low, medium, high doses, celecoxib, SC79, and XFC-H with SC79.
Osteoarthritis was induced in all but control group using collagenase.
Histological analysis of cartilage using specific staining methods.
Serum biomarkers were measured using ELISA for various cytokines and proteins.
Western blot analysis was used to detect PI3K, p-PI3K, AKT, p-AKT expression in cartilage.
Model group showed increased inflammatory markers IL-1β, IL-6, TNF-α, TGF-β1, TRACP-5b, and decreased OPG, BALP, IGF-1 compared to control.
XFC treatment significantly reduced inflammatory markers and restored OPG, BALP, and IGF-1 levels compared to model group.
SC79 treatment showed worse inflammation and cartilage damage compared to XFC-induced improvements.

Resumen

目的探究新风胶囊（XFC）通过PI3K/AKT信号通路对骨关节炎（OA）大鼠炎症及骨代谢的影响。方法 64只SD大鼠等分为8组：对照（Control）组，模型（Model）组，XFC低、中、高剂量（XFC-L、XFC-M、XFC-H）组，塞来昔布（Celecoxib）组，SC79（AKT激动剂）组，XFC-H+SC79组；对除Control组外大鼠以胶原蛋白酶制备OA模型。番红固绿、阿利辛蓝染色进行软骨组织学分析；ELISA检测血清中TGF-β1、TNF-α、IL-1β、IL-6、TRACP-5b、IGF-1、OPG、BALP表达；免疫印迹检测软骨中PI3K、p-PI3K、AKT、p-AKT 蛋白表达。结果与Control组比较，Model组软骨表面粗糙，软骨细胞及细胞外基质减少，潮线破坏；IL-1β、IL-6、TNF-α、TGF-β1、TRACP-5b、p-PI3K、p-AKT表达升高（PPPP 结论 XFC调节PI3K/AKT信号通路减轻OA大鼠炎症及骨代谢紊乱，改善软骨损伤。

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