Saline–alkali soils suffer from severe deficiencies in available phosphorus, and externally added phosphorus is readily immobilized by metal ions in the soil. Therefore, activating inorganic phosphorus in the soil represents a significant challenge. In this study, 35 salt–alkali-tolerant bacteria were isolated from rhizosphere soils (pH 9.20–9.68). Three phosphate-solubilizing strains (HA2, HPA5, and KA1) capable of growing under severe saline–alkali stress conditions (pH 10, 5% NaCl) and possessing multiple plant growth-promoting traits (nitrogen fixation, potassium solubilization, siderophore production, and IAA secretion) were screened and co-cultured to form the microbial consortium HHK. It was hypothesized that this consortium might exhibit synergistic effects, resulting in significantly higher phosphorus solubilization capacity compared to individual strains. The results showed that under saline–alkali stress, the phosphate solubilization capacity of HHK (484.59 ± 15.79 mg/L) was significantly higher than that of any single strain (285.59 ± 12.60 mg/L). Non-targeted metabolomics and enzyme assays indicated that HHK solubilizes P via organic acids (e.g., citric, L-malic acid) and synergistically modulates core metabolic pathways, including ABC transport, TCA cycle, and glycolysis, alleviating oxidative damage and maintaining cellular homeostasis. Short-term soil incubation confirmed that HHK significantly increased available phosphorus (53.67%) and soil fertility, indicating its potential as a biofertilizer.
Wang et al. (Sat,) studied this question.