Results: Plasma creatinine level was elevated significantly at 24 hrs to 7 d post-IR compared to that in sham-operated rats.Increased urine output and decreased urine osmolality were observed at 24 hrs to 7 d after IR. mRNA expression level of a hypoxia marker, Glut1 (a downstream gene of Hif), was increased at 2 and 24 hrs after IR.Nqo1, an oxidative stress marker (a downstream gene of Nrf2), mRNA level peaked at 24 hrs post-IR.Grp78 mRNA, an ER stress marker, was increased only at 6 hrs post-IR.TGFb mRNA, a fibrosis marker, was gradually increased over 6 hrs to 7 d.In relation to increased urinary solutes excretions in ischemic AKI, Slc6a19 (a neutral amino acid transporter), Slc34a1 (phosphate transporter), and Sglt2 (glucose transporter) mRNAs were significantly decreased at 24 hrs post-IR.Furthermore, results from RNA seq analyses supported the abovementioned results. Conclusion:These results indicate that hypoxic response ( 24 hrs), ER stress response (6 hrs), and oxidative stress response (24 hrs) occur in the early phase of renal IR.Given that decreases in amino acid, glucose, and phosphate transporters 24 hrs after renal IR, the mechanisms underlying reductions of these transporters include hypoxia, oxidative stress, ER stress, or their combination.This study may contribute to the development of an appropriate drug therapy against AKI.I have no potential conflict of interest to disclose.I did not use generative AI and AI-assisted technologies in the writing process.
Saipidin et al. (Wed,) studied this question.