What question did this study set out to answer?

This research aims to assess the prevalence and genetic diversity of apple mosaic virus in apple and hazelnut trees in Serbia.

April 1, 2026Open Access

Occurrence and diversity of apple mosaic ilarvirus in apple and hazelnut in Serbia

Puntos clave

This research aims to assess the prevalence and genetic diversity of apple mosaic virus in apple and hazelnut trees in Serbia.
Tested 220 trees (150 apple, 70 hazelnut) from seven locations in Serbia.
Used ELISA test to detect apple mosaic virus presence.
Conducted RT-PCR with two primer pairs for further analysis of virus isolates.
Detected apple mosaic virus in 11 apple samples (7.3%) and 6 hazelnut samples (8.5%).
Mosaic symptoms were observed on leaves of affected trees.
Serbian apple isolates showed 98.66 ‒ 99.02% nucleotide identity with isolates from India and Canada.

Resumen

Two hundred and twenty trees (150 apples and 70 hazelnuts) from seven locations in Serbia were tested for the presence of Ilarvirus ApMV (apple mosaic virus). ELISA test confirmed the presence of ApMV in 11 apples (7.3%) and six hazelnuts (8.5%) samples, with typical and severe mosaic symptoms on the leaves. ELISA-positive samples were further tested by RT-PCR with two different primer pairs. One primer pair was efficient for the RT-PCR detection of ApMV isolates from apple, and the other one for isolates from hazelnut. Sequence analysis of amplified PCR fragments of the selected isolates confirmed that Serbian ApMV isolates from apple share the highest nucleotide identity (98.66 ‒ 99.02%) with isolates from India and Canada.

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Cite This Study

VASILIJEVIĆ et al. (Thu,) studied this question.

synapsesocial.com/papers/69ccb59f16edfba7beb8771e https://doi.org/https://doi.org/10.5513/jcea01/27.1.4927

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