The CYP11B1 promoter was significantly hypomethylated in cortisol-producing adenomas compared to adjacent unaffected adrenal tissue, leading to CYP11B1 overexpression and cortisol overproduction.
Observational (n=20)
No
DNA hypomethylation of the CYP11B1 promoter, which is further reduced by PRKACA or GNAS somatic mutations, plays a key role in CYP11B1 overexpression and cortisol overproduction in cortisol-producing adenomas.
valor p: p=<0.01
Adrenocortical hormone excess, due to primary aldosteronism (PA) or hypercortisolemia, causes hypertension and cardiovascular complications. In PA, hypomethylation of aldosterone synthase (CYP11B2) is associated with aldosterone overproduction. However, in hypercortisolemia, the role of DNA methylation of 11β-hydroxylase (CYP11B1), which catalyzes cortisol biosynthesis and is highly homologous to CYP11B2, is unclear. The aims of our study were to determine whether the CYP11B1 expression was regulated through DNA methylation in hypercortisolemia with cortisol-producing adenoma (CPA), and to investigate a possible relationship between DNA methylation and somatic mutations identified in CPA. Methylation analysis showed that the CYP11B1 promoter was significantly less methylated in CPA than in adjacent unaffected adrenal tissue and white blood cells. Furthermore, in CPA with somatic mutations in either the catalytic subunit of protein kinase A (PRKACA) or the guanine nucleotide-binding protein subunit alpha (GNAS) gene, the CYP11B1 promoter was significantly hypomethylated. In addition, DNA methylation reduced CYP11B1 promoter activity using a reporter assay. Our study results suggest that DNA methylation at the CYP11B1 promoter plays a role in the regulation of CYP11B1 expression and cortisol production in CPA, and that somatic mutations associated with CPA reduce DNA methylation at the CYP11B1 promoter. © 2017 The Author(s).
米田 et al. (Fri,) conducted a observational in Hypercortisolemia with cortisol-producing adenoma (n=20). Cortisol-producing adenoma (CPA) vs. Adjacent unaffected adrenal tissue (AUAT) and non-functioning adrenal tumors was evaluated on CYP11B1 promoter methylation level (p=<0.01). The CYP11B1 promoter was significantly hypomethylated in cortisol-producing adenomas compared to adjacent unaffected adrenal tissue, leading to CYP11B1 overexpression and cortisol overproduction.