Exploring the mechanism of italicPingchong Jiangni Formula/italic （平冲降逆方） in treating endometriosis： an integrated study based on network pharmacology and experimental validation

Puntos clave

• The research aims to explore the potential molecular mechanisms of Pingchong Jiangni Formula in treating endometriosis using network pharmacology and experimental validation.
• Active ingredients and target interactions were identified using TCMSP and BATMAN-TCM databases.
• Disease targets related to endometriosis were obtained from GeneCards, OMIM, and TTD databases.
• Core therapeutic targets were selected using STRING database and Cytoscape software.
• GO and KEGG enrichment analyses were performed via DAVID database.
• Molecular docking experiments were conducted using AutoDock, validated by animal experiments.
• Identified 147 effective active components and 212 intersecting targets for the formula.
• Key targets include IL6, TNF, AKT1, TP53, ALB, IL-1β, and ESR1.
• Biological functions mainly involved positive regulation of RNA polymerase II promoter transcription.
• Molecular docking revealed strong binding activities of quercetin, abietic acid, crocin, and kaempferol with core targets.
• Animal experiments showed significant reduction in the volume of ectopic lesions and improvement in tissue pathology.