Mechanism of italicHuangqi Baijiang Yiren Decoction/italic （黄芪败酱薏仁汤） in ameliorating inflammatory injury of HT-29 cells via the miR-21-5p/SOCS1/JAK1/STAT6 signaling pathway

Puntos clave

• This research aims to explore the mechanism of Huangqi Baijiang Yiren Decoction in improving inflammatory injury in HT-29 cells.
• Induced inflammation in HT-29 cells using TNF-α.
• Divided experimental groups into control, model, and varying doses of HBYD and mesalazine.
• Measured inflammatory factor levels using ELISA.
• Assessed mRNA levels through qPCR for target genes.
• Conducted Western blot analysis for protein expression of specific markers.
• Inflammatory markers IL-6, IL-18, IL-1β, and TNF-α levels increased significantly in model group compared to control.
• High-dose HBYD significantly reduced levels of inflammatory factors and normalized protein expressions related to cell junctions.
• miR-21-5p overexpression showed opposing effects on SOCS1, JAK1, and STAT6 pathway components.