Abstract 653: Rapid drug sensitivity testing of passage-zero patient brain tumor tissues to inform preclinical and clinical decisions: results from a clinical feasibility study.

Abstract Functional precision medicine (FPM) offers a promising path forward in neuro-oncology, where genomic profiling alone often fails to predict therapeutic response. To bridge this gap, we developed the Screening Live Cancer Explants (SLiCE) platform, a rapid ex vivo drug screening assay that functionally tests passage-zero patient brain tumor tissues engrafted atop living organotypic brain slice cultures (OBSCs). With an assay time of just four days, SLiCE preserves key tumor characteristics not maintained in vitro, including genomic fidelity, growth, invasion, and treatment response, with higher engraftment rates and faster assay speeds than in vivo models. Our standard cryopreservation workflow enables reproducible, iterative, and on-demand testing of a single zero-passage specimen banked in multiple replicate aliquots, setting SLiCE apart from organoid and precision-cut tumor explant models. Here, we describe results from our actively accruing clinical feasibility study (NCT05978557), where we successfully engrafted and tested 35 of 36 diverse brain tumor specimens on SLiCE, achieving our study’s primary endpoint ahead of schedule. SLiCE produced multi-parametric drug sensitivity scores (DSSs), each normalized to off-target toxicity, for all samples within a clinically actionable 28-day window. Across 530 experiments, we generated 142 DSSs from unique drug-tumor combinations, forming a reference library for future benchmarking. We then further analyzed a subset of IDH-WT glioblastoma tumor specimens in which SLiCE DSSs correlated with patient response to temozolomide (AUC = 0.875, p = 0.0175) and overall survival (R2 = 0.73). Additionally, this study validated surgically aspirated tumor tissue as a genomically, transcriptomically, and functionally similar tumor source compared to the standard, manually excised remnant tumor sample approved by clinical pathology. Collecting this often-discarded tumor source increased the mass of tumors accrued by nearly 5-fold and enabled collection from 11 additional patients, significantly increasing tumor tissue for downstream testing on SLiCE. These findings establish SLiCE as a scalable, clinically relevant platform for FPM in brain cancer, with potential to guide individualized treatment decisions and accelerate preclinical drug development. Citation Format: Breanna Mann, Nichole Artz, Adebimpe Adefolaju, Alain Valdivia, Xiaopei Zhang, Rajaneekar Dasari, Caroline Stockwell, Morrent Thang, Allison Murray, Noah Bell, Andrew Buckley, Rami Darawsheh, Kerry Fitzgerald, Jonathan Williams, Hardik Parikh, Shuang Gao, Jie An, Yvette Rodriguez, Daniel Metzger, Collin Parrow, Dylan Riley, Robert Seager, Stephanie B. Hastings, Taylor Jensen, Shakti Ramkissoon, Dominique Higgins, Yasmeen Rauf, Scott Elton, Kimberly Hamilton, Jeremy Wang, Albert Baldwin, Shawn Hingtgen, David Kram, Andrew Satterlee. Rapid drug sensitivity testing of passage-zero patient brain tumor tissues to inform preclinical and clinical decisions: results from a clinical feasibility study abstract. In: Proceedings of the American Association for Cancer Research Annual Meeting 2026; Part 1 (Regular Abstracts); 2026 Apr 17-22; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2026;86(7 Suppl):Abstract nr 653.