Evaluation of patients before and after CABG revealed activation of platelet apoptosis through an extrinsic receptor-dependent signaling pathway, indicating significant metabolic changes.
Observational (n=30)
Does CABG alter platelet apoptosis and activation in patients with coronary artery disease?
CABG induces significant changes in platelet metabolic processes and apoptosis via an extrinsic receptor-dependent pathway, which may be associated with postoperative complications.
Cardiovascular diseases have been one of the leading causes of death worldwide for over 30 years and coronary artery disease occupies a prominent place among them. Predicting the long-term outcome after coronary artery bypass graft (CABG) is still a challenging task; however, since platelets are directly involved in the course of the disease, their functional status can help predict postoperative complications. The objective was to evaluate the dynamics of platelet apoptosis before and after CABG. The study involved 30 patients with a scheduled CABG suffering from coronary artery disease. Flow cytometry, Western blot and polymerase chain reaction were used to evaluate apoptosis and the activation of platelets. Changes in platelet membranes and the depolarization of mitochondrial membranes were observed, as well as the expression of active caspase 8 and BCL-2, indicating the activation of apoptosis through an extrinsic receptor-dependent signaling pathway. The data obtained suggest significant changes in platelet metabolic processes, which could play a crucial role in the development of coronary artery disease and atherosclerosis as well as being associated with postoperative complications.
Metelkin et al. (Mon,) llevaron a cabo una observacional en enfermedad arterial coronaria (n=30). Se evaluó la cirugía de bypass coronario (CABG) vs. la línea base preoperatoria en la dinámica de la apoptosis y activación plaquetaria. La evaluación de los pacientes antes y después de CABG reveló activación de la apoptosis plaquetaria a través de una vía de señalización dependiente de receptores extrínsecos, indicando cambios metabólicos significativos.