Can We Trust Ex Vivo Mechanical Testing of Fresh–Frozen Cadaveric Specimens? The Effect of Postfreezing Delays

This study indicates that when using fresh-frozen cadaveric bone in biomechanical tests to simulate the orthopaedic clinical setting, specimen exposure time should be considered. The timing of testing should be kept constant between specimens to allow for a proper comparison. Furthermore, for fresh--frozen cadavers, the physical properties of bone may be detrimentally affected in biomechanical testing that exceeds the 50-hour time point after removal from the freezer.