Quantitative Trait Loci Analysis for Anthracnose Resistance in a Population Derived From Andean Varieties Bukoba and Kijivu of Common Bean (Phaseolus vulgaris L.)

ABSTRACT Anthracnose (ANT) caused by the fungus ( Colletotrichum lindemuthianum ) is a major disease of common bean ( Phaseolus vulgaris L.). The objective of this study was to identify genomic regions and candidate genes for resistance to Races 19, 38, 51, 167, 263, 1050 and 1105 of C. lindemuthianum in a population of recombinant inbred lines (RILs) derived from Andean genotypes Bukoba and Kijivu. A total of 155 F 5:7 RILs and parents were evaluated for resistance to seven ANT races using artificial inoculations. The population was genotyped with 11,292 SNPs, and quantitative trait loci (QTL) analysis was conducted. A total of six QTL for resistance to seven races were identified on chromosomes Pv01 (ANT1.1 BK ), Pv02 (ANT2.1 BK ), Pv04 (ANT4.1 BK ), Pv05 (ANT5.1 BK ), Pv10 (ANT10.1 BK ) and Pv11 (ANT11.1 BK ). The R 2 values for these QTL ranged from 5.2% (ANT5.1 BK ) to 70.1% (ANT1.1 BK ) suggesting that resistance to the seven races was controlled by both major‐ and minor‐effect QTL. Of the six QTL, ANT5.1 BK was novel while the remaining overlapped with previously identified loci. ANT1.1 BK , which provided resistance to two races (1050 and 1105), overlapped with Co‐1 while ANT4.1 BK , which provided resistance to Races 19, 51, 183 and 263 overlapped with loci Co‐3 , Co‐15 , Co‐16 , Co‐y and Co‐z . Clusters of resistance genes with NB‐ARC domain were identified as candidate genes for QTL ANT4.1 BK and ANT11.1 BK . Pyramiding ANT1.1 BK and ANT4.1 BK would provide effective resistance to Races 19, 51, 167, 263, 1050 and 1105.