Broad-spectrum antimicrobial activity of volatile organic compounds from endophytic Pseudomonas putida BP25 against diverse plant pathogens

Black pepper endophytic Pseudomonas putida BP25 produced diverse antimicrobial volatile organic compounds having potential for plant disease management. Chemically synthesised volatiles such as 2, 5-dimethyl pyrazine; 2-methyl pyrazine; dimethyl trisulphide; 2-ethyl 5-methyl pyrazine; and 2-ethyl 3, 6-dimethyl pyrazine showed inhibitory activity against oomycete pathogens, Phytophthora capsici fungal pathogens, Rhizoctonia solani, Colletotrichum gloeosporioides, Athelia rolfsii, Gibberella moniliformis bacterial pathogen, Ralstonia pseudosolanacearum and plant parasitic nematode, Radopholus similis. Among them, dimethyl trisulphide completely inhibited oomycete and fungal pathogens as well as R. similis at a concentration of 2.65 µg cm−3 under in vitro conditions. Pyrazines suppressed Phytophthora lesions on shoot cuttings of black pepper upon in planta volatile treatment. Dimethyl trisulphide was the only compound that exhibited soil fumigant activity against P. capsici, R. solani and A. rolfsii (6.25 µg cm−3), C. gloeosporioides and G. moniliformis (12.5 µg cm−3), and R. similis (50 µg cm−3). Altogether, endophytic Pseudomonas putida BP25 and its volatile organic compounds offer an alternative strategy for eco-friendly disease management in agriculture.