Ambient air pollution is a global problem linked to various diseases, including neurodegenerative disorders. Using a mobile exposure system, we investigated the molecular changes in human olfactory cleft mucosal (hOM) cells from healthy individuals and Alzheimer's disease (AD) patients exposed to real-world ambient air. Samples were exposed at the air-liquid interface in a low-pollution Background site and a moderately polluted (within recommended limits) Industrial site. We assessed immune response-related molecules, lipid peroxidation and global RNA expression. Leukemia-inhibitory factor (LIF) increased in healthy, but not AD samples at the Industrial locality, while lipid peroxidation levels remained unchanged. Compared with a blank control, significant enrichment of biological processes and KEGG pathways occurred only in Industrial site samples. In healthy cells, neurodegeneration-related pathways were significantly affected, whereas AD samples showed enhanced inflammation-related responses. These findings suggest that even pollution within recommended limits can induce molecular changes potentially contributing to neurodegenerative disease development. • Real-world air pollution exposure was studied in primary olfactory mucosal cells • Effects compared in primary cells from healthy donors and Alzheimer’s patients • Industrial pollution altered neurodegeneration-related pathways in healthy cells • Increased levels of leukemia-inhibitory factor in healthy samples were found • In Alzheimer's samples, inflammation-related response was detected
Rössner et al. (Wed,) studied this question.