Base editing in rice using nuclease‐deactivated CRISPR/Cas‐SF01

Puntos clave

• The research aims to explore adenine and cytosine base editing in rice using dCas-SF01.
• Utilized nuclease-deactivated Cas9 variant (dCas-SF01) for editing.
• Employed the 35S-CmYLCV-U6 composite promoter for expression.
• Applied PAM-relaxed variant SF01-IKRR to target 5'-NTN PAMs.
• Achieved average editing efficiency of 33.3%.
• Successfully introduced targeted base substitutions at multiple loci in rice.