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The increasing microbial drug resistance in recent times has necessitated the search for an alternative antimicrobial agents derived from natural sources. Chromolaena odorata L. (Asteraceae) is one of such natural sources that has been reported to possess healing properties. In this study, the phytochemical constituents and antimicrobial properties of C. odorata leaf extract were investigated. Methodology: The leaves of C. odorata were collected from Babcock University garden, authenticated, prepared and extracted following standard procedures with methanol and ethyl-ether as extraction solvents. Phytochemical screening was carried out according standard protocol while antimicrobial screening was performed according to agar well diffusion method on the following organisms: Pseudomonas aeruginosa (ATCC19582), Shigella flexneri (KZN), Klebsiella pneumoniae (ATCC 10031), Enterococcus faecalis (ATCC 29212), Enterococcus cloacae (ATCC
Odutayo et al. (Tue,) studied this question.