ABSTRACT Three bile acid derivatives, 8a , 8b , and 8c, were synthesized from 1,1‐di(methylsulfanyl)‐2‐nitro ethylene (6) and C3‐amino bile methyl ester ( 5a , 5b , and 5c ), using aqueous medium, followed by hydrolysis, and confirmed with FT‐IR, NMR, and mass spectroscopy techniques. The newly synthesized facial amphiphilic bile acid derivatives showed a good range of antimicrobial action against various multidrug‐resistant, such as Gram‐positive, Gram‐negative, antibacterial, and antifungal microorganisms. Preliminarily, the zone of inhibition (ZOI) was determined by the agar well diffusion method. The results reveal that bile acid‐derived compounds showed the most effective ZOI against both Gram‐positive and Gram‐negative bacteria, as well as fungi. According to the ZOI outcomes, the minimum inhibitory concentration (MIC) was assessed using the broth microdilution (LB) method for six Gram‐positive and Gram‐negative bacterial strains. Among six bacterial strains, Salmonella typhi , Staphylococcus aureus , and Escherichia coli illustrated great inhibition effects against the reference molecule ciprofloxacin. All three compounds showed good efficiency in the MIC inhibition at a 50 µg/mL concentration. E. coli , S. aureus (Gram‐positive), and Klebsiella pneumoniae (Gram‐negative) pathogens possessed good activity against the reference molecule ciprofloxacin at lower concentrations of 3.125 and 6.25 µg/mL MIC values. In addition, synthesized compounds were found to have the greatest scavenging activity in DPPH, ABTS, and FRAP assays against the reference compound ascorbic acid.
Ramakrishnan et al. (Wed,) studied this question.