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High-Resolution Genotyping by Amplicon Melting Analysis Using LCGreen | Synapse

June 1, 2003Open Access

High-Resolution Genotyping by Amplicon Melting Analysis Using LCGreen

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Resumen

High-resolution melting analysis of PCR products amplified in the presence of LCGreen can identify both heterozygous and homozygous sequence variants. The technique requires only the usual unlabeled primers and a generic double-stranded DNA dye added before PCR for amplicon genotyping, and is a promising method for mutation screening.

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Cite This Study

Wittwer et al. (Sun,) studied this question.

synapsesocial.com/papers/69ea1695186fc979e9a81a57 https://doi.org/https://doi.org/10.1373/49.6.853

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Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context:

1Amplicon Melting Analysis with Labeled Primers: A Closed-Tube Method for Differentiating Homozygotes and Heterozygotes2003 · 365 citations
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4[31] Detection and localization of single base changes by denaturing gradient gel electrophoresis1987 · 936 citations
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